ABSTRACT
Purpose: The document outlines the preferred methods for collecting blood from laboratory animals and blood collection volume and frequency limits. Blood collection for experimental purposes must comply with researchers of the Drug research institute (DRI) approved protocol, including approved collection techniques, volumes, and frequencies. The Department of Pharmacology researchers train investigators in various collection techniques. The researcher may collect blood for veterinary care purposes using accepted clinical techniques ensuring volumes collected do not adversely affect animal health. Blood Collection Limits: The DRI limits one time survival blood collection to 7.5% of an animal’s blood volume in most circumstances. Serial blood sampling limit vary by species, strain and frequency of blood collection. The DRI may require monitoring for anemia (using assays such as hematocrit and/or serum protein levels) when repeated collection of larger volumes are required. Blood collected for diagnostics or other veterinary procedures must be considered when evaluating total volume available for experimental use. In all cases blood collection volumes should be limited to the minimum volume that will allow for successful experimentation or diagnostics.
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The genus Iris belongs to the family Iridaceae and comprises of over 300 species and fifteen species of genus Iris are found in Mongolia. Iris has long history of use in various indigenous systems of medicine as alternative aperients, stimulant, cathartic, diuretic, gall bladder diseases, liver complaints, dropsy, purification of blood, venereal infections, fever and bilious infections and for a variety of heart diseases. Rhizomes of Iris are rich source of secondary metabolites and most of these metabolites are reported to possess anticancer, antiplasmodial, anticholinesterase, enzyme inhibitor and immunomodulatory properties. Approximately more than two hundred compounds have been reported from the genus Iris, which includes flavones, isoflavones, glycocides, benzoquinones, triterpinoids, stilbene glycosides and organic acids. In this article, we reviewed the published results of phytochemical and pharmacological studies of some Iris species which are grown in Mongolia.
ABSTRACT
Background: In recent years the number of diseases that arise from compromised immune system has been steadily such as autoimmune and immunity expressing cleseases, research has been going on order to deverlop a highly effective medicine made from natural resources, with low risk of increasing toxicity and side effects that can be used to treat or prevent these diseases. Throughout the world, there are a number of plants that have been identified with immune boosting ability and the following plants Astragalus mongolicus Bunge , Salsola laricifolia Turcz, Oxytropis pseudoglandulosa Gontsch, Inula helenium that have been proven to support the immune system and grow in Mongolia were selected for a phytochemical study. Goal: An objective was set forth to identify a proper extragent (extraction solvent) to extract the biologically active compounds found in Astragalus mongolicus Bunge , Salsola laricifolia Turcz, Oxytropis pseudoglandulosa Gontsch, Inula helenium plants known to stimulate immune function. Results : Salsola laricifolia’s coumarin content was the highest or 0.33% when extracted with 60% alcohol, the flavonoid content was 0.56% when 55%, 60% alcohol was used as the extragent, 60% alcohol is determined to be an appropriate extragent. Astragalus mongolicus’s coumarin and flavonoid composition was quite high in 25% alcohol, specifically it contained 0.04% coumarin and 0.20% flavonoid. Inula helenium’s 50% alcohol extract contained 0.25% coumarin, 25% alcohol extract had a flavonoid content of 0.59% and 50% alcohol is determined to be proper extragent in future research Oxytropis pseudoglandulosa’s 50% alcohol contained 0.65% coumarin and 0.7% flavonoid, therefore 50% alcohol will be used to extract this plant for furthrer research